Download Atlas on the Human Testis: Normal Morphology and Pathology by Ivan Krhen M.D., Ph.D., Andreja Vukasović M.D. (auth.), PDF

By Ivan Krhen M.D., Ph.D., Andreja Vukasović M.D. (auth.), Davor Ježek (eds.)

Atlas at the Human Testis: common Morphology and Pathology offers histological illustrative fabric from paraffin and semi-thin sections of the human testis that are typically utilized in the overview of testicular morphology, permitting an early detection of carcinoma in situ and extra complicated pathological adjustments of the testicular parenchyma. The early detection of melanoma in situ is predicated at the cautious morphological research of the biopsy and immunohistochemistry (IHC). consequently, this atlas includes specified descriptions of IHC equipment in addition to glossy molecular organic equipment resembling DNA microarrays and proteomics and complicated microscopy concepts concerning the testicular biopsy.

Adequate overview of the testicular biopsy results in excessive medication premiums of testicular neoplasms which might be used as a foundation to effectively deal with infertility in males.

Atlas at the Human Testis: general Morphology and Pathology is a invaluable reference instrument as a way to entice andrologists, urologists, pathologists, scientific embryologists, in addition to reproductive biology scientists.

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Extra info for Atlas on the Human Testis: Normal Morphology and Pathology

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For the planned TESE/ICSI attempt, the TESE is performed as follows. Frozen samples are retrieved from the liquid nitrogen and immediately transferred to a water bath (37 °C). The sperm-freezing medium (in our case, MediCult) is replaced with 1 mL of pre-warmed (37 °C) spermpreparation medium (MediCult) and incubated 32 A. Vukasović et al. for 1 h. 2 mg of trypsin inhibitor (SigmaAldrich). After 2 h of incubation, the samples are macerated with sterile needles and centrifuged for 10 min at 800 × g and 37 °C.

Com During this period, Leydig cells of the fetal type undergo regression and dedifferentiation. They become smaller, vacuolated, and, in the end, D. 1007/978-1-4471-2763-5_5, © Springer-Verlag London 2013 55 M. Kos and T. Leniček 56 spindle shaped, blending into the interstitium. Some of these cells also may undergo apoptosis. The tunica albuginea thickens steadily, and the tunica vasculosa forms, as do the interlobular septa that are invariably present after 25–28 weeks’ gestation. The future seminiferous tubules a b Fig.

Although immunohistochemical (IHC) analysis is not done routinely, for the purpose of this chapter and for a better understanding of fetal development, certain markers were used (see the figures). 10–20 Weeks’ Gestation During the 11th gestational week, proliferation and maturation of Leydig cells begin, inducing the transformation of gonocytes to prespermatogonia and resulting in changes in microscopic appearance. By 14 weeks’ gestation, Leydig cells constitute half the volume of the testis, separating the tubules by a distance of one to four tubular diameters.

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