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By Randy J. Horwitz MD, PhD, Robert F. Lemanske Jr. MD (auth.), Phil Lieberman MD, John A. Anderson MD (eds.)

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Williams PB, Dolen WK, Koepke JW, Selner JC. Immunoassay of specific IgE: use of a single point calibration curve in the modified radioallergosorbent test. Ann Allergy 1992; 69: 48-52. 4 Environmental Allergens Scott H Sicherer, MD and Peyton A. Eggleston, MD CONTENTS INTRODUCTION OUTDOOR ALLERGENS INDOOR ALLERGENS ACKNOWLEDGMENTS SUGGESTED READING INTRODUCTION Allergens are small-mol-wt proteins capable of inducing IgE antibody and triggering an allergic response. In our outdoor and indoor milieu, these allergenic proteins (along with a larger number of nonallergenic proteins) are carried on vectors, such as pollen grains or house dust particles, which may become airborne.

Antibody. Following another wash to remove the unbound, labeled anti-IgE, the quantity of labeled IgE is measured and converted into units of IgE by comparison to a standard curve. A variety of commercial assays are available, and most are accurate to a concentration of <5 IU/mL (12 ng/mL) ofIgE. Serum concentrations of IgE vary widely in normal individuals (Table 3). IgE levels are very low at birth and gradually rise, peaking in the second decade of life, followed by a slow decline into old age.

Serum concentrations of IgE vary widely in normal individuals (Table 3). IgE levels are very low at birth and gradually rise, peaking in the second decade of life, followed by a slow decline into old age. Although the geometric mean values are relatively low, there is a very large 95% confidence interval at all ages (Table 3). 4 ng of IgE. The new Systeme International (SI) specifies that IgE be reported as micrograms per liter ()lg/L) with two significant digits (XX x IOn). Relationship of Total IgE to Allergic Disease Most studies have shown that total serum IgE concentrations tend to be higher in allergic adults and children compared to nonallergic individuals of similar ages.

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